Part:BBa_K4621000:Design

EctOperon is a cluster of genes encoding four enzymes that synthesize 5-Hydroxyectoine

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 3227
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 3227
  Illegal NotI site found at 571
  Illegal NotI site found at 1456
  Illegal NotI site found at 1774
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 753
  Illegal XhoI site found at 927
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 3227
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 3227
  Illegal NgoMIV site found at 788
  Illegal NgoMIV site found at 1194
  Illegal NgoMIV site found at 1200
  Illegal NgoMIV site found at 1491
  Illegal NgoMIV site found at 2540
  Illegal NgoMIV site found at 2732
  Illegal NgoMIV site found at 3242
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 376
  Illegal BsaI.rc site found at 556
  Illegal BsaI.rc site found at 1338
  Illegal BsaI.rc site found at 1866
  Illegal BsaI.rc site found at 2121
  Illegal BsaI.rc site found at 2211
  Illegal BsaI.rc site found at 2286
  Illegal SapI site found at 414
  

Design Notes

The GC content will be taken into account when designing the primers.

Source

This part is derived from SCUT-3 genome.

References

[1] Garcia-Estepa R et al.Sys Appl Microbiol, 2006, 29 (8) :626-633